Richard Keys and Andy Gray have been stood down from duty for the televised match between Bolton and Chelsea, Sky Sports have confirmed in a statement.
The Presenters have been removed from 'Monday Night Football' after making comments off-air about Premier League official Sian Massey.
Barney Francis, managing director of Sky Sports, said: "It has been made clear to each of them that their comments were totally unacceptable."
Keys and Gray commented on Massey's appointment for the game between Wolves and Liverpool at Molineux on Saturday, when they believed their microphones were switched off.
They concurred that female officials "don't know the offside rule", with Keys adding: "I can guarantee you there'll be a big one today. (Liverpool manager) Kenny (Dalglish) will go potty."
Keys also went on to remark on comments made by West Ham vice-chairman Karren Brady in The Sun on Saturday morning about the level of sexism in football by saying: "See charming Karren Brady this morning complaining about sexism? Yeah. Do me a favour, love."
Francis continued: "I have spoken directly to both Richard Keys and Andy Gray this morning.
"Those views are inexcusable, entirely inconsistent with our ethos as a business and employer, and will rightly offend many of our customers, our people, and the wider public.
"They are inexcusable from anyone at Sky, regardless of their role or seniority.
"We have dealt with this matter by taking immediate disciplinary action.
"As with any employee it would not be right to go into detail on those proceedings.
"However, they have been clearly warned about their behaviour and reminded of their responsibilities.
"In addition, Richard and Andy will not be involved in any way with tonight's live Monday Night Football."