The Central Florida Zoo and Botanical Gardens is a 116-acre (47 ha) zoo and botanical garden located north of Orlando, Florida in Sanford. As a not-for-profit organization, it is a leader in conservation, providing experiences that inspire actions on behalf of wildlife. The Zoo has been an accredited member of the Association of Zoos and Aquariums (AZA) since 1986.

In 1923, the Central Florida Zoo (then called the Sanford Municipal Zoo) opened its gates for the first time with only a small collection of animals that were donated by the local fire department. In 1941, it was relocated to new facilities where Sanford City Hall now stands. It was moved to its current location on July 4, 1975.

With the support from a group of dedicated citizens, and the donation of a 106-acre piece of land from Seminole County, the Central Florida Zoological Park opened at its current location along Lake Monroe, with Jack Hanna as Director. In 1986 the Central Florida Zoo became accredited by the Association of Zoos and Aquariums (AZA). In 2005, The Wayne M. Densch Discovery Center and ZooLab opened. The buildings feature classroom and hands-on instructional space, as well as a multi-purpose banquet room available for private meeting and event rentals. In 2007, the Zoo officially announced its botanical garden status and became the Central Florida Zoo and Botanical Gardens. The same year, the Wharton-Smith Tropical Splash Ground opened.

Within the zoo, a small carousel and splash park can be found. Giraffe feedings and rhino encounters are available for an extra cost. The Little Florida Railroad, a 16 in (406 mm) gauge ridable miniature railway with a G-16 streamliner locomotive originally built in 1951, began operating at the zoo in 2003.

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