The star sang in memory of the late singer, who was murdered in 1995, and was joined on stage by Selana's siblings Suzette and A.B., as well as her widow Chris Pérez.
The medley, honouring the 20th anniversary of the later musician's death, also featured more memorable tracks from her back catalogue including 'Amor Prohibido', 'I Could Fall In Love' and 'No Me Queda Mas'.
Despite being applauded for the spectacular performance at the Bank United Centre in Coral Gables, Miami, Jennifer had other matters to deal with during the evening as she was confronted by her ex-husband Marc Anthony and his new wife, Shannon De Lima.