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Synopsis: With the increasing worldwide incidence of obesity and theresulting increase in metabolic disorders such as diabetes,cardiovascular diseases and some cancers, an understanding of themultitude of factors that contribute to obesity is crucial forresearchers to develop effective treatments. This collectionof overviews and protocols provides the reader with an introductionto the major features of obesity research, including the interplaybetween energy balance, hormones and neurobiological networks. Anoverview is provided which describes the animal models of obesitymost commonly utilized in research. A standardized laboratoryprotocol for producing diet?]induced obesity (DIO) in both mouseand rat is provided as are protocols that describe how dietformulation can be modified to generate mouse models of humanmetabolic pathologies. Protocols are also provided for testing theeffects of dietary manipulations, caloric restriction and potentialtherapeutics.
This e-book - a curated collection from eLS, WIREs, andCurrent Protocols - offers a fantastic introduction to thefield of obesity research for students or interdisciplinarycollaborators.
Table of Contents:
Introduction
Energy Balance, Obesity and Type 2 Diabetes
eLS
M. Daniel Lane
Neurobiology of Overeating
eLS
Eric Stice and Kyle S. Burger
Primary Hormones and Receptors Studied in ObesityResearch
Obesity Hormones in Health and Disease
eLS
Emrah Caylak
Central Dopaminergic Circuitry Controlling Food Intake andReward: Implications for the Regulation of Obesity
WIREs Systems Biology and Medicine
Zivjena Vuceti and Teresa M. Reyes
Protocols
Overview of Animal Models of Obesity
Current Protocols in Pharmacology
Thomas A. Lutz and Stephen C. Woods
Diet-Induced Models of Obesity (DIO) in Rodents
Current Protocols in Neuroscience
Didier Bagnol, Hussien Al-Shamma, Dominic Behan, Kevin Whelan andAndrew J. Grottick
Dietary Manipulation of Mouse Metabolism
Current Protocols in Molecular Biology
Jerome N. Feige, Marie Lagouge and Johan Auwerx